Category: Genetic Engineering.

What is ARMS-PCR or allele-specific PCR?

The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA (PCR amplification of specific alleles)…

Role of nested PCR in microbial identification

The nested qPCR or the nested RT-PCR gives great power to this technique which can be a helpful method for…

What is nested PCR?

In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the non-specific binding with the help…

Protocol for inverse PCR

In the beginning, before doing any wet lab work, design the primers complementary to the know DNA regions using the…

Inverse PCR

Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the primers which amplify the flanking…

Maxam-Gilbert Sequencing: What Was It, and Why

In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or chemical sequencing) method and the Sanger…

Clone Library Screening

The result of any cloning experiment that begins with total DNA from a specific source is a library of clones. Thus cloning…

Prokaryotic Expression Vectors

The first vector we will consider is the pUC family of vectors Figure 3.4.1: pUC vector Although it is not typically used for…

cDNA and Genomic Libraries

Isolation of corresponding genetic information Instead of synthesizing a desired gene, can we used the amino acid information to directly…

Types and Functions of Plasmids

Resistance Plasmids Also referred to as antimicrobial resistance plasmids, resistance plasmids are a type of plasmids that carry genes that…

What are Plasmids?

Essentially, plasmids are small, circular molecules of DNA that are capable of replicating independently. As such, they do not rely…

Linkers And Adapter