Genetic Engineering 8 min read
Colony PCR
Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally this has been done with restriction enzyme…
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What is Hot Start PCR?
Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during…
Genetic Engineering 5 min read
How to do ARMS-PCR?
The process of the ARMS PCR is simple and very effective. No harmful radiolabelling is involved in the ARMS allele-specific…
Genetic Engineering 3 min read
What is ARMS-PCR or allele-specific PCR?
The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA (PCR amplification of specific alleles)…
Genetic Engineering 2 min read
Role of nested PCR in microbial identification
The nested qPCR or the nested RT-PCR gives great power to this technique which can be a helpful method for…
Genetic Engineering 5 min read
What is nested PCR?
In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the non-specific binding with the help…
Genetic Engineering 5 min read
Protocol for inverse PCR
In the beginning, before doing any wet lab work, design the primers complementary to the know DNA regions using the…
Genetic Engineering 5 min read
Inverse PCR
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the primers which amplify the flanking…
Genetic Engineering 3 min read
Maxam-Gilbert Sequencing: What Was It, and Why
In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or chemical sequencing) method and the Sanger…
Genetic Engineering 3 min read
Clone Library Screening
The result of any cloning experiment that begins with total DNA from a specific source is a library of clones. Thus cloning…
Genetic Engineering 11 min read
Prokaryotic Expression Vectors
The first vector we will consider is the pUC family of vectors Figure 3.4.1: pUC vector Although it is not typically used for…
Genetic Engineering 7 min read
cDNA and Genomic Libraries
Isolation of corresponding genetic information Instead of synthesizing a desired gene, can we used the amino acid information to directly…