UPDATES

What is nested PCR?

In the nested PCR, the specificity of the PCR reaction is enhanced…

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July 14, 2021

Protocol for inverse PCR

In the beginning, before doing any wet lab work, design the primers…

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July 14, 2021

Inverse PCR

Here, the unknown DNA sequence adjacent to the known template DNA is…

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July 14, 2021

Maxam-Gilbert Sequencing: What Was It, and Why

In the mid-1970s, two methods were developed for directly sequencing DNA: the…

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July 14, 2021

Clone Library Screening

The result of any cloning experiment that begins with total DNA from…

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July 14, 2021

Prokaryotic Expression Vectors

The first vector we will consider is the pUC family of vectors Figure 3.4.1: pUC…

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July 14, 2021

cDNA and Genomic Libraries

Isolation of corresponding genetic information Instead of synthesizing a desired gene, can…

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July 14, 2021

Types and Functions of Plasmids

Resistance Plasmids Also referred to as antimicrobial resistance plasmids, resistance plasmids are…

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July 14, 2021

What are Plasmids?

Essentially, plasmids are small, circular molecules of DNA that are capable of…

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July 14, 2021

Linkers And Adapter

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July 14, 2021