Genetic Engineering
What is ARMS-PCR or allele-specific PCR?
The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA (PCR amplification of…
July 14, 2021 · Rajil TLAuthor: Rajil TL
Rajil TL is a SenseCentral contributor focused on tech, apps, tools, and product-building insights. He writes practical content for creators, founders, and learners—covering workflows, software strategies, and real-world implementation tips. His style is direct, structured, and action-oriented, often turning complex ideas into step-by-step guidance. He’s passionate about building useful digital products and sharing what works.
Genetic Engineering
Role of nested PCR in microbial identification
The nested qPCR or the nested RT-PCR gives great power to this technique which can be a helpful…
July 14, 2021 · Rajil TLGenetic Engineering
What is nested PCR?
In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the non-specific binding with…
July 14, 2021 · Rajil TLGenetic Engineering
Protocol for inverse PCR
In the beginning, before doing any wet lab work, design the primers complementary to the know DNA regions…
July 14, 2021 · Rajil TLGenetic Engineering
Inverse PCR
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the primers which amplify…
July 14, 2021 · Rajil TLGenetic Engineering
Maxam-Gilbert Sequencing: What Was It, and Why
In the mid-1970s, two methods were developed for directly sequencing DNA: the Maxam-Gilbert sequencing (or chemical sequencing) method…
July 14, 2021 · Rajil TLGenetic Engineering
Clone Library Screening
The result of any cloning experiment that begins with total DNA from a specific source is a library of clones.…
July 14, 2021 · Rajil TLGenetic Engineering
Prokaryotic Expression Vectors
The first vector we will consider is the pUC family of vectors Figure 3.4.1: pUC vector Although it is not typically…
July 14, 2021 · Rajil TLGenetic Engineering
cDNA and Genomic Libraries
Isolation of corresponding genetic information Instead of synthesizing a desired gene, can we used the amino acid information…
July 14, 2021 · Rajil TL
Genetic Engineering
Types and Functions of Plasmids
Resistance Plasmids Also referred to as antimicrobial resistance plasmids, resistance plasmids are a type of plasmids that carry…
July 14, 2021 · Rajil TL