Principle of centrifugation
CENTRIFUGE Centrifugation is a technique used for the separation of particles using a centrifugal field. The particles are suspended in liquid medium and placed in a centrifuge tube. The tube…
Chromatography
Chromatography is derived from Greek terms “chromo= color & gram=bands.” Hence as the name indicates, in chromatography, there is the formation of colored bands. These bands are indicative of different components in the sample. In the Initial…
Colorimeters
Introduction A colorimeter is a light-sensitive device used for measuring the transmittance and absorbance of light passing through a liquid sample. The device measures the intensity or concentration of the…
Gluconeogenesis
Gluconeogenesis is the process of synthesizing glucose from non-carbohydrate sources. The process takes place mainly in the liver and limited extent in the kidney and small intestine under some conditions. It is…
Recombinant DNA
Recombinant DNA, which is often shortened to rDNA, is an artificially made DNA strand that is formed by the combination of two or more gene sequences. This new combination may…
DNA Damage and Repair
Damage to cellular DNA is involved in mutagenesis and the development of cancer. The DNA in a human cell undergoes several thousand to a million damaging events per day, generated…
Hybrid Orbitals
Hybrid orbitals are the result of a model which combines atomic orbitals on a single atom in ways that lead to a new set of orbitals that have geometries appropariate to form…
Transcriptome
For all that we can learn from the genome, it’s the other ‘omic disciplines that spell out an organism’s biology. Want to know what proteins a cell makes? Try proteomics.…
Central Dogma
The ‘Central Dogma’ is the process by which the instructions in DNA are converted into a functional product. It was first proposed in 1958 by Francis Crick, discoverer of the…
