What are the differences between PCR, RT-PCR, qPCR, and RT-qPCR?
Polymerase chain reaction (PCR) is a relatively simple and widely used molecular biology technique to amplify and detect DNA and RNA sequences. Compared to traditional methods of DNA cloning and…
The role of PCR in single cell analysis
The polymerase chain reaction (PCR) has a wealth of applications in research, but perhaps one of its most challenging roles in single cell analysis, amplifying the DNA from just a single cell,…
Colony PCR
Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish the same thing in less…
What is Hot Start PCR?
Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot…
How to do ARMS-PCR?
The process of the ARMS PCR is simple and very effective. No harmful radiolabelling is involved in the ARMS allele-specific PCR. The procedure of ARMS PCR is divided into 4…
What is ARMS-PCR or allele-specific PCR?
The allele-specific PCR also called as an ARMS- PCR (amplification refractory mutation system) or PASA (PCR amplification of specific alleles) or AS-PCR used to detect the SNPs. More specifically, it…
Role of nested PCR in microbial identification
The nested qPCR or the nested RT-PCR gives great power to this technique which can be a helpful method for the phylogenetic analysis and identification of different pathogens. The technique…
What is nested PCR?
In the nested PCR, the specificity of the PCR reaction is enhanced by reducing the non-specific binding with the help of the two sets of primer. The specificity is the…
Protocol for inverse PCR
In the beginning, before doing any wet lab work, design the primers complementary to the know DNA regions using the computational software. The detail information of how to design primer…
Inverse PCR
Here, the unknown DNA sequence adjacent to the known template DNA is amplified using the primers which amplify the flanking (unknown) region near the know DNA template. Quite difficult to…
