Microbiology Laboratory Safety Rules and Procedure
Safety in a microbiology laboratory is important in the prevention of infection as Microbiology laboratory cultures, manipulates, and uses virulent and/or potentially pathogenic microorganisms. In addition to microorganisms, there are…
Endospore Staining: Principle, Procedure and Results
When vegetative cells of certain bacteria such as Bacillus spp and Clostridium spp are subjected to environmental stresses such as nutrient deprivation, they produce metabolically inactive or dormant form-endospore. Formation of endospore circumvent the…
FLAGELLA STAIN
INTENDED USE Hardy Diagnostics Flagella Stain is recommended for use in detecting the presence and arrangement of flagella on the bacterial cell. SUMMARY Bacterial flagella, due to their narrow diameter,…
Acid-Fast Stain- Principle, Procedure, Interpretation and Examples
It is the differential staining techniques which was first developed by Ziehl and later on modified by Neelsen. So this method is also called Ziehl-Neelsen staining techniques. Neelsen in 1883 used Ziehl’s…
Gram Staining: Principle, Procedure and Results
Gram staining method, the most important procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884. Gram staining is still the cornerstone of bacterial identification and taxonomic division.…
Acid Dyes
Today we know a large number of aromatic and aliphatic compounds. Hydrocarbons are most common examples of aliphatic compounds whereas aromatic compounds are cyclic compounds with delocalized electrons which make…
Differential Staining Techniques
Viewing Bacterial Cells The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells…
What is a Limitation of Using Electron Microscopes to View Specimens?
How does electron microscopy work? Three main factors underlie the scientific principle of microscopy: magnification, illumination, and resolution. Resolution is perhaps the central tenet of microscopy in that it defines the…
Difference Between AFM and STM
AFM and STM AFM refers to Atomic Force Microscope and STM refers to Scanning Tunneling Microscope. The development of these two microscopes is considered a revolution in the atomic and…
Atomic Force Microscopy, a Powerful Tool in Microbiology
INTRODUCTION Understanding the functions of microbial cell surfaces requires knowledge of their structural and physical properties. Electron microscopy has long been recognized as a key technique in microbiology to elucidate…
