Acid Dyes
Today we know a large number of aromatic and aliphatic compounds. Hydrocarbons are most common examples of aliphatic compounds whereas aromatic compounds are cyclic compounds with delocalized electrons which make…
Differential Staining Techniques
Viewing Bacterial Cells The microscope is a very important tool in microbiology, but there are limitations when it comes to using one to observe cells in general and bacterial cells…
What is a Limitation of Using Electron Microscopes to View Specimens?
How does electron microscopy work? Three main factors underlie the scientific principle of microscopy: magnification, illumination, and resolution. Resolution is perhaps the central tenet of microscopy in that it defines the…
Difference Between AFM and STM
AFM and STM AFM refers to Atomic Force Microscope and STM refers to Scanning Tunneling Microscope. The development of these two microscopes is considered a revolution in the atomic and…
Atomic Force Microscopy, a Powerful Tool in Microbiology
INTRODUCTION Understanding the functions of microbial cell surfaces requires knowledge of their structural and physical properties. Electron microscopy has long been recognized as a key technique in microbiology to elucidate…
Difference Between Scanning Electron Microscope and Transmission Electron Microscope
The world of the very small first opened itself to the eyes of mankind in 1595 when Zaccharias Janssen invented the first modern light microscope. This type of microscope uses…
Difference Between Optical and Electron Microscope
Optical vs. Electron Microscopes Many students are instantly plagued when placed in front of a microscope. How does it operate, or what type of microscope is it? Even if they…
SEM and TEM: what’s the difference?
Electron microscopes have emerged as a powerful tool for the characterization of a wide range of materials. Their versatility and extremely high spatial resolution render them a very valuable tool…
Scanning Electron Microscopy (SEM)
What is Scanning Electron Microscopy (SEM) A typical SEM instrument, showing the electron column, sample chamber, EDS detector, electronics console, and visual display monitors. The scanning electron microscope (SEM) uses…
Transmission Electron Microscope
A Transmission Electron Microscope (TEM) utilizes energetic electrons to provide morphologic, compositional and crystallographic information on samples. At a maximum potential magnification of 1 nanometer, TEMs are the most powerful microscopes. TEMs…
